同时感知炎症和致病菌，按需生产三联治疗蛋白，同时双开关安全模块防止细菌污染——精准、安全、高效。
图：FeloVance 三联疗法治疗猫口炎概念图
六大核心创新，覆盖猫口炎治疗全链条
同时监测炎症信号和致病菌信号，覆盖猫口炎两大病因
IFN-ω（抗病毒）+ LL-37（抗菌）+ TGF-β1（免疫调节），一个表达盒产出三种蛋白
单启动子驱动三个蛋白等比例表达，避免复杂调控
温度感应+pH感应，双重保障
感知模块整合基因组（稳定），输出模块放在高拷贝质粒（高效）
唾液链球菌K12，口腔天然益生菌，自带一定的黏附能力
设计双信号感知模块：P_NF-κB+TLR4/MD-2
设计输出模块：IFNW1-P2A-CAMP-P2A-TGFB1三联疗法
设计双安全开关：温度感应-cspA 5'UTR+pH感应-Pasr
合成优化后的基因片段：IFNW1、CAMP、TGFB1
构建治疗质粒：pSB1C3-PT7-三联疗法
整合安全模块至K12基因组
实验1：炎症感知验证（TNF-α刺激→GFP表达）
实验2：三联疗法表达验证（Western Blot）
实验3：安全模块验证（温度/pH条件培养）
实验4-6：抗菌/抗病毒/免疫调节功能验证
循环1→2：密码子优化提高表达效率
循环2→3：调整RBS序列平衡三个蛋白表达量
循环3→4：优化cspA 5'UTR提高温度敏感性
下一轮：引入Hsa黏附蛋白增强定植
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